발표논문

제목 [청혈단] 청혈단의 유전자 발현 조절을 통한 내피세포에서의 항염증 효과
청혈단의 유전자 발현 조절을 통한 내피세포에서의 항염증 효과
Chunghyul-dan acts as an anti-inflammatory agent in endothelial cells by regulating gene expression
저자 강동경희대병원 의료진 : 박정미(공동), 박성욱(공동), 고창남 (공동)
타기관 의료진 : 박현용(교신저자), 정우상(1저자), 조진구(공동), 인경민(공동), 김종민(공동), 조기호(공동), 문상관(공동), 김경욱(공동), 피재호(공동), 박상규(공동), 정윤화(공동)
저널명 Animal Cells and Systems 2010;14(4)
게재일 2010.12.10.
논문소개
-초록정리
Chunghyul-dan (CHD) is a combinatorial drug known to exert anti-inflammatory effects in endothelial cells. In this study, we employed global transcriptional profiling using cDNA microarrays to identify molecular mechanisms responsible for the anti-inflammatory activity of CHD in endothelial cells. An analysis of the microarray data revealed that transcript levels of monocyte chemotactic protein-1 (MCP-1), vascular cell-adhesion molecule-1 (VCAM-1) and activated leukocyte cell-adhesion molecule were dramatically altered in CHD-treated endothelial cells. These changes in gene expression were confirmed by RT-PCR, Western blotting and ELISA. Chronic CHD treatment also appeared to decrease MCP-1 secretion, probably as a result of decreased MCP-1 expression. In addition, we determined that chronic CHD treatment inhibited lipopolysaccharide-stimulated adhesion of THP-1 leukocytes to endothelial cells. The inhibitory effect of CHD on LPS-stimulated adhesion resulted from down-regulation of VCAM-1 expression. Transmigration of THP-1 leukocytes through endothelial cells was also inhibited by chronic CHD treatment. In conclusion, CHD controls a variety of inflammatory activities by regulating MCP-1 and VCAM-1 gene expression.
페이지 URL http://www.tandfonline.com/doi/abs/10.1080/19768354.2010.525824

목록