발표논문

제목 [성장] 사인이 흰쥐의 장골 성장에 미치는 영향
사인이 흰쥐의 장골 성장에 미치는 영향
Amomum villosum induces longitudinal bone growth in adolescent female rats
저자 Sun Haeng Lee, Ji Young Kim, Hocheol Kim, Seul Ki Park, Cho Young Kim, Sun Yong Chung, Gyu Tae Chang
저널명 J Tradit Chin Med
게재일 2012 September 15; 32(3): 453-458
논문소개 Abstract
OBJECTIVE : This study was conducted to evaluate the effect of Amomum villosum on longitudinal bone growth.

METHODS : Adolescent female Sprague-Dawley rats were divided into 3 groups and treated for 4 days: control (distilled water, p.o.), recombinant human growth hormone (rhGH; 100 μg/kg, s.c.), and A. villosum (500 mg/kg, p.o.) groups. On day 3, tetracycline (20 g/kg, i.p.) was injected for growth plate identification. On days 2, 3 and 4, 5-bromo-2'-deoxyuridine (BrdU) (50 mg/kg, i.p.) was injected to label proliferating cells. On day 5, tibias were dissected and fixed in 4% paraformaldehyde, dehydrated, and sectioned for immunohistochemistry and histomorphometry.

RESULTS : The rate of bone growth in the A. villosum and rhGH groups increased to (410 ± 44) and (389 ± 46) μm/day (P<0.01), respectively, as compared with the control (330.7 ± 34.7) μm/day. The thickness of the growth plates also increased to (591 ± 37) and (598 ± 32) μm, respectively, as compared with the control (524±89) μm (P<0.001).
The number of BrdU-positive cells in the chondrocytes of the A. villosum and rhGH groups was also significantly higher (126±24) and (143±18) cells/mm2, respectively) than in the control (109 ± 25) mm2 (P<0.05). Insulin-like growth factor-1 and bone morphogenetic protein-2 in the A. villosum and rhGH groups were highly expressed in the growth plate as compared with the control samples, indicating increased bone formation.

CONCLUSIONS : A. villosum could be used to treat growth retardation during adolescence.

Keywords : Amomum villosum; Growth plate; Bone Development; Recombinant human growth hormone
페이지 URL http://www.sciencedirect.com/science/article/pii/S0254627213600540

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