발표논문

제목 [청혈단] 청혈단은 사람의 내피세포에서 NOS mRNA 발현을 촉진하고, VCAM-1 mRNA의 발현을 억제한다.
청혈단은 사람의 내피세포에서 NOS mRNA 발현을 촉진하고, VCAM-1 mRNA의 발현을 억제한다.
Chunghyuldan activates NOS mRNA expression and suppresses VCAM-1 mRNA expression in human endothelial cells.
저자 강동경희대병원 의료진 : 박성욱(1저자), 고창남(공동), 배형섭(공동)
타기관 의료진 : 김영석(교신저자), 정우상(공동), 문상관(공동), 조기호(공동), 최성길(공동)
저널명 Canadian Journal of Physiology and Pharmacology 2005;83(12):1101-8.
게재일 2006.01.25.
논문소개
-초록정리
Chunghyuldan (CHD), a combinatorial drug that has antihyperlipidemic and anti-inflammatory activities, has been shown to improve arterial stiffness and inhibit stroke recurrence in clinical study. To understand the molecular basis of CHD's clinical effects, we explored its effect on cell proliferation and expression of nitric oxide synthase (NOS) and vascular cell adhesion molecule (VCAM-1) in human umbilical vein endothelial cells (HUVECs). Cell number counting and [3H]thymidine incorporation assay demonstrated that nontoxic doses of CHD have an inhibitory effect on DNA synthesis and suppress cell cycle progression of HUVECs. CHD treatment led to a marked induction of NO production through up-regulation of NOS mRNA expression in a dose- and time-dependent manner, whereas it suppressed VCAM-1 expression. CHD inhibition of VCAM-1 expression was totally blocked by pretreatment with the NO synthesis inhibitor L-NMMA, whereas pretreatment with the NO donor DETA-NO further decreased VCAM-1 level in CHD-treated HUVECs, indicating that VCAM-1 regulation by CHD is mediated through increased NO synthesis by CHD. In addition, TNF-alpha-mediated VCAM-1 activation was substantially impeded by CHD treatment. Collectively, our data suggest that anti-inflammatory or anti-hyperlipidemic effects of CHD might be associated with its ability to activate NO production and suppress VCAM-1 expression in human endothelial cells.
페이지 URL http://www.nrcresearchpress.com/doi/abs/10.1139/y05-106?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed&#.VnZzrbaLS1s

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